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This week, we will be performing a DpnI digest on our OE-PCR cloning reaction, then transforming it into chemically competent DH5 E. coli by heat shock, and plating them out on LB ampicillin plates. DpnI Digest, Ligation, and Transformation of Chemically Competent E. coli 1. Add 1 L of DpnI enzyme to your PCR reaction from last week. μ 2. Incubate in the PCR machine using the heat block incubation function, at 37°C for 15 minutes. 3. Ask a demonstrator for an aliquot DH5 chemi ...

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Tags: Nepal Kathmandu Biology Biology Advanced Biology University of Kathmandu Biology 


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