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HLSC 2P09 LAB MANUAL

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You will be provided with MRC5 human lung fibroblast cells which have been grown in cell culture medium (DMEM) containing glucose and other necessary nutrients and supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution. The cells adhere and grow in cell culture flasks in an incubator at 37º C, 5% CO2. These cells have been trypsinized, to detach them from the flask and stored in 1.5mL microtubes prior to your lab section.  
1. Ensure the haemocytometer and glass plate have been cleaned using 70% ethanol and a Kimwipe. Prepare the haemocytometer by placing the glass slide on top of the counting grid. 2. Using your p20 pipet, first mix the cells in your microtube by pipetting up and down several times. Collect 10µL of cells in media using your pipet. 3. Holding the pipet on a 45-degree angle, place the pipet tip in the groove of the counting grid and slowly release the cells. 4. Ensure that your cells in media cover the entire counting grid area. If not, then move the glass slide around slightly to spread out the cells. 5. Place the grid under the microscope and using the 10x setting adjust the focus so that the cells on the grid can be seen. Referring to the figure below, count the cells which appear in the 4 corner squares. Note that when cells are on the edge of the square you will only count 2 of the edges

HLSC 2P09 LAB MANUAL

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Tellus molestie nunc non blandit massa enim nec dui. Tellus molestie nunc non blandit massa enim nec dui. Ac tortor vitae purus faucibus ornare suspendisse sed nisi. Pharetra et ultrices neque ornare aenean euismod. Pretium viverra suspendisse potenti nullam ac tortor vitae. Morbi quis commodo odio aenean sed. At consectetur lorem donec massa sapien faucibus et. Nisi quis eleifend quam adipiscing vitae proin sagittis nisl rhoncus. Duis at tellus at urna condimentum mattis pellentesque. Vivamus at augue eget arcu dictum varius duis at. Justo donec enim diam vulputate ut. Blandit libero volutpat sed cras ornare arcu. Ac felis donec et odio pellentesque diam volutpat commodo. Convallis a cras semper auctor neque. Tempus iaculis urna id volutpat lacus. Tortor consequat id porta nibh.

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More HLSC 2P09 LAB MANUAL : Questions & Answers

Q

Business Management

Assignment Title: ‘3D printing represents a completely new era in which long established operational techniques will prove to be completely outdated and this, in turn, will have serious ramifications in terms of unemployment.’Critically examine this statement. Referencing: Please ensure ...

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CIS3002/CIS5302

For CIS3002 students all tasks relate to the Case Study which will be provided – except for CIS5302 students who are expected to find their own “real-world” case study   As an indication of length - Tasks worth 10 marks are expected to take approximately one page of work as ...

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2208HSL International Food and Beverage Management

Your task is to critically analyse two fine dining or up-market restaurants of two, 4-5 star hotels or resorts (i.e. one restaurant from each hotel or resort) in the city of the Perth, Western Australia. A 4-5 star stand-alone restaurant may be used if your research deems it more suitable for compar ...

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Corporate Law

Part A 1. Go to the following website http://www.austlii.edu.au/ and locate this case: Peter Smythe v Vincent Thomas [2007] NSWSC 844 (3 August 2007) Explain briefly how you found it. 2 marks 2. What particular circumstances led Rein AJ to doubt the credibility of the defendant’s version of ev ...

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