Each experimental aim is designed to answer one hypothesis. Experiment 1: To confirm the reduction of cell proliferation by counting the proliferating cells that were marked with BrdU-marker Experiment 2: To confirm the increase of apoptotic activity by counting the number of cells that were undergoing apoptosis, which can be visualized using TUNEL visualization technique Experiment 3: To measure the level of neurotrophic factors (BDNF and GDNF protein) using ELISA analysis
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