Outline Of Report Structure
Title of Your Project
The role of platelet heterogeneity in cardiovascular disease. (If expert think after reading my aim, hypothesis, rational of the research to change the tittle to something more relevant he can do it)
Aim of Research: studying pro-coagulant platelets. Questioning What intracellular and extracellular signals determine whether a platelet aggregates or becomes pro-coagulant? How do pro-coagulant platelets contribute to thrombosis? Can we prevent platelets becoming pro-coagulant, and would this reduce thrombosis?
Rationale: During thrombosis, platelets adhere to a ruptured atherosclerotic plaque and become activated. Most activated platelets aggregate together but an important subpopulation instead become pro-coagulant. They then interact with the coagulation cascade and generate a burst of thrombin that is responsible for forming a stable, occlusive thrombus.
Hypothesis: Platelets are crucial to normal haemostasis but play critical role in arterial thrombosis and myocardial infarction, which is a major cause of death
Samples required: Cell culture, tissue culture, Bio-marker, cell signalling
Techniques: sing a variety of approaches involving flow cytometry, fluorescence imaging, protein biochemistry and proteomics, PCR, western blotting, or anything expert think itâ€™s better to set up new experiment you need to use only one technique.
Format of data: Table, graphs, charts, electrophoresis gel, Images, Data of research, diagrams.
Additional details: cardiovascular disease, heart attack, Ca2+, cell signalling, cell heterogeneity, cell death
Abstract (150-200 words)
Summary of your proposal. All the major points relating to your proposed research should be covered here, in a very concise, focused narrative.
Introduction and rationale (500-600 words)
This should provide a solid background to the project grounded in research literature. If your proposed research is about a disease or a protein or a technique, introduce it here. Use the background information to explain how your proposed research differs from that already carried out and justify your proposed work. Remember that readers of research proposals usually know less about the research area than the authors, so make sure the background information and your ideas are introduced in a logical way that is easy to follow. You should include references in this section, perhaps from text books but more likely from journal research articles. See examples of how references are used in introduction sections in the journal articles you read. Be concise and focused- this should not include everything you have read about your subject of interest!
Aims & Hypothesis (~50 words)
Explain clearly and concisely what you are trying to find out and what you think the result will be.
Experimental Design (600-800 words)
What is your research strategy going to be? Outline the methods and techniques that you propose to use and state their purpose in the context of your overall aim. This section should include a flow diagram outlining the samples you would collect, how they would be processed in the laboratory and how the results of each technique would be interpreted to answer your research question.
Make sure that the data to be generated will answer your question/test your hypothesis i.e. donâ€™t use a protein analysis technique if your aim is to study DNA unless you clearly explain how the results can be interpreted to provide the required information about the DNA of interest. Give an overview of the techniques to be used and the materials required. This should include numbers of human volunteers or animal/cell/sample requirements if appropriate. We require enough experimental detail to know that you understand the principles of the techniques you are proposing to use and what they involve but you are not expected to include the very specific details such as volumes or concentrations. Suggest why these techniques are the most appropriate to perform the study and include a health and safety statement in this section. You will need to use references to support your choice of techniques and/or to refer to details that you donâ€™t have space to include. Ensure that this section primarily consists of cell and/or molecular biology techniques- clinical studies alone for example are unlikely to provide you with sufficient opportunity to demonstrate your skills and understanding relating specifically to this unit.
Ethics (150-200 words)
ALL research has potential ethical implications. Outline those associated with your proposed research and describe how they are related to your hypothesis and aims and/or to your experimental methodology. Are the ethical issues raised justifiable in terms of the research proposed? Are there alternatives that could be used that you have rejected? If so, why? Have you proposed any non-optimal details mainly to avoid using less ethical alternatives? Do you think your proposed research would be permitted in this country or anywhere else and/or would you need specific approval from a professional body or similar?
Results (400-500 words)
Explain the format of data expected from the experiments performed and what you would expect to see based on your hypothesis. This could be a prediction of both positive and negative results, e.g. what will you see if the expression of â€˜gene Xâ€™ isnâ€™t related to tumour formation as well as if it is. This section should not include actual predictions of specific values- you are unlikely to be able to predict to that level of detail. However, you should be able to demonstrate that you understand the format of the results likely to be generated by your proposed methodology (e.g. gel images following PCR or western blotting, microscopy images following immunohistochemistry) and how you can interpret and analyse the data in order to test your hypothesis. Donâ€™t forget to discuss output for every technique mentioned in the Experimental Design section and vice versa.
Outcomes & Conclusion (~100 words)
What will the outcomes of your research be? What questions will have been answered? Who will benefit from it? Is there potential for further work or related studies?
Refer to text books and research journals that you can find in the library or through the LRC electronic resources and use the Harvard system of referencing in the format requested by the Journal of Cell Science, available here: http://jcs.biologists.org/content/manuscript-prep#ref. It is better to and you will get more credit for using journal articles, however if you use an internet source, it must be from an appropriate organisation (e.g. University or perhaps a Society for your chosen topic) and should be authored, not Wikipedia or other similar sites. Please ensure that your references relate to the relevant documents and their authors/publishers etc., NOT just the place where you found them. All the references should be year 2010-2015, In total should be 12-15 references.
Figures & Tables
All students should include a flow diagram as requested in the experimental design section as a minimum. In addition, tables and/or figures can be used to illustrate details in the text if appropriate, for example to clarify the metabolic pathway being studied. All figures should be clearly labelled, should have a full legend and should be referred to directly in any relevant text. Wherever possible, figures & tables should be your own work (i.e. not cut & pasted or photocopied). An exception would be for example a crystal structure of a protein or any other similarly complex figure which we would not reasonably expect a student to draw. However, if you do use images that are not your own, you must reference these correctly and state that they are not you own e.g. â€˜reproduced fromâ€¦.â€™
Things need to be follow carefully:
This is your opportunity to study an area of cell and/or molecular biology that interests you in greater depth. It will allow you to read the scientific literature about your area of interest, to identify areas where further research can be done and to propose the appropriate methodology to perform your study. You must predict the likely outcomes of your study and discuss any health and safety or ethical issues that may be raised by the research.
A key difference between this assignment and those you are probably more familiar with is that you are required to devise some new experiments capable of generating new data (not entirely new techniques!). Therefore, it is not sufficient to describe experiments you have read about in journal articles or just research and describe lots of general information about a particular gene or disease etc. Your task here is to demonstrate that you understand how the techniques you have learnt about work, the types of data that they generate, their advantages and disadvantages compared to alternatives and how they can be applied to a research question of your choice.
â€¢ Demonstrate a critical understanding of the cellular and molecular biology techniques including genomics, proteomics, nanotechnology and protein engineering.
â€¢ Collate and independently evaluate significant volumes of scientific data, information and ideas to propose new hypotheses which could form the basis of new research projects; create original responses to problems that expand or redefine existing knowledge or develop new approaches to changing situations. Evidence independent, self-directed learning and originality in problem solving when completing assignments.
Please follow the write up guideline and outline of project research. Make sure it's deliver on time and be aware of the plagiarism please. Last time my work was delayed for 1 day make sure this time it's shouldn't be any delay. Expert can choose his own journal and article relevant to the work so he/she no need to wait for my reply. I have posted all the relevant information there is nothing i can provide anymore. Make sure expert set up new experiment by using old established techniques. Project outline will explain my tittle of the project, aim, rational, hypothesis and etc. So read the project outline first and then read write up guideline and then start writing. Thank You
Please find the attached files and follow them top to bottom. I am looking for first class work. This is should be master level work. So please don't do any low quality work as this is for my masters course. Thank You