The movement of the cells to the opposite poles of the cell due to splitting of the newly-copied chromosomes also known as daughter chromosomes is the stage of mitosis after metaphase where chromosomes that are newly replicated gets split is known as anaphase. The re-formation of nucleus and the segregation of chromosomes occurs due to the overall maximum condensation during the late stage of anaphase also occur during anaphase.
The initiation of anaphase begins with the anaphase promoting complex marking an inhibitory chaperone, which are proteins named securing for destruction by regulatory protein. With the destruction of securin, an enzyme separase is unleashed breaking down cohesion, a protein, which is responsible for holding sister chromatids together. After the completion of anaphase, the cell then goes to the next phase, which is telophase.
There are two distinct motions based on which anaphase is characterized. The two phases of anaphase are termed as Anaphase A and Anaphase B. The combination of forces that act on chromatids are of various nature but the primary force is exerted centrally. Centromeres are attached with the midpoint of chromosomes using kinetochores. The movement of external forces is generated due to action of external forces.
Anaphase B has distinctive mechanisms based on forces generated due to various actions. The interpolar microtubules join with the equator of dividing cells and push each other which causes the centrosome to move far apart. Astral microtubules start at the centrosomes join with each other closer to the cell membrane. These movements are generated due to the motor proteins known as dyneins or kinesins.
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