Securing Higher Grades Costing Your Pocket? Book Your Assignment at The Lowest Price Now!

Effect Of Amylase On Starch Add in library

49 Download3 Pages 632 Words

Question:

Write a report about the Effect of Amylase on Starch?
 
 

Answer:

The experiment was performed to determine the activity of salivary amylase, where the enzyme converts the starch to maltose. The enzyme hydrolyzes the-(1-4) glycosidic linkage of the starch polymers (Amira et al., 2012) into maltose and dextrin. The enzymatic action is influenced by pH and temperature (Amira et al., 2012). The amylases isolated from diverse sources (including plants, animals, and microbes) play a vital role in carbohydrate metabolism. In addition, due to associated low cost, the enzyme is being widely used in industrial production for food processing and fermentation etc. However the action of the enzyme varies from source to source. Hence the efficacy of the enzyme should be determined. The effect of amylase can be determined using iodine as an indicator. In brief the starch is treated with known concentration of amylase under identical conditions. The starch gets hydrolyzed proportionally to that of action of amylase. The starch show blue colour in presence of iodine solution and disappears with the degradation (conversion to smaller units) of starch. Hence the method, iodine-starch combination is used for the quantitative assessment (Xiao, et al., 2006). Amylase also shows a partial degradation of starch to amyloamylose and erythroamylose (Freeman & Hopkins, 1936). As soon as the conversion is in progress the blue colour gets transformed to clear zone due to hydrolysis of starch to maltose. The results of zone of clearance obtained from the experiment are shown in Table-1 and Fig-1.

Table-1: Effect of Amylase on digestion of starch based on zone of clearance

Amylase conc (%)

Zone of clearance/m2

0

0

0.5

40

1

80

1.5

136

2

96

2.5

128


The results suggesting that there exists haphazard pattern of results. The experimental values are not on straight-line for 2 and 2.5% amylase solutions. The zone of clearance is marginally linear to that of concentrations of amylase solution standalone (r=0.88); whereas the elimination of terminal points yielded a straight line (r=1). The correlation coefficient (r) is used as an indicator to express the linearity. The results indicate that the zone of clearance values resulted for the concentrations, 2 % and 2.5 are not in linear. Therefore these two points could be considered as the anomalies among the values.

 

Precautions to avoid the errors

Specific volumes of the samples should be pipetted accurately and trasnfrred to the glas tubes. The experiemntal considitons should be identical to all the samples. Ideally the samples should be exposed to a temperature of 37o C. In the current case, there were few errors that include improper pippeting, droping temperare from 36o C to 29o C and spillage of the samples. These are the probable reasosn for showing descreency of the results. Therefore the experiemnts showed haphzardous results. The experiemtnal errors can be minimized by care ful experimentation. The experiemnt looks to be sensitive to changes in the temperature. In addtion the method is sesnitive to changes in pH. Therefore the method further can be improved by maintaining a cosntant pH utilizing buffers and temperature for all the experiemnts. Else an advanced method based on electrophoresis (Martinez et al., 2000) may be used to yield consistent and reproducible results.

 

References

FREEMAN GG & Hopkins RH (1936) The mechanism of degradation of starch by amylases: Kinetics of action of α- and β-amylases on starch, its components and partial degradation products. Biochemical J 30(3) p. 446-450

MARTINEZ TF., ALARCON FJ., DIAZ-LOPEZ M & MOYANO FJ (2000) Improved detection of amylase activity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with copolymerized starch. Electrophoresis. 21(14) p. 2940-3.

XIAO, Z., STORMS, R & TSANG, A. (2006) A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities. Anal Biochem. 351(1) p. 146-8.

AMIRA E-F., MOHAMMED AD., AHMED E-S & NOHA O (2012) Starch and microbial α-Amylases: from concepts to biotechnological applications, carbohydrates-comprehensive studies on glycobiology and glycotechnology, Retrieved from https://www.intechopen.com/books/carbohydrates-comprehensive-studies-on-glycobiology-and-glycotechnology/starch-and-microbial-amylases-from-concepts-to-biotechnological-applications

OR

With time, MyAssignmenthelp.com has become one of the best college essay writing services. Our all rounded services give students the confidence to overcome assignment related issues. Our services are fast enough to meet most urgent deadlines. We are capable of delivering fast essay writing services with the help of our specially designed provisions and writers' teams. Our urgent essay help services guarantee most rapid delivery of assignment solutions. Some of our most popular essay services are application essay help, exploratory essay help, literary review essay help and argumentative essay help.

Most Downloaded Sample of Management

271 Download1 Pages 48 Words

Toulin Method Of Argumentation

You are required to write a researched argument essay that convinces persuades the reader of your position / stance. This is an academic, researched and referenced do...

Read More Tags: Australia Arlington Management Management University of New South Wales Management 
202 Download9 Pages 2,237 Words

Consumer Behavior Assignment

Executive Summary The purpose of this report is to elaborate the factors which are considered by individuals before selecting an occupation. Choosing an occupati...

Read More Tags: Australia Arlington Management Management University of New South Wales Management 
367 Download13 Pages 3,112 Words

Internet Marketing Plan For River Island

Introduction With the increase enhancement in the field of technology, it has been considered essential by the businesses to implement such technology in their b...

Read More Tags: Australia Arlington Management Management University of New South Wales Management 
325 Download9 Pages 2,203 Words

Strategic Role Of HR In Mergers & Acquisitions

Executive Summary In a merger & acquisition, role of an HR has emerged as a very critical function. At each stage of merger and acquisition process, HR plays a s...

Read More Tags: Australia Arlington Management Management University of New South Wales Management 
353 Download7 Pages 1,521 Words

Relationship Between Knowledge Management, Organization Learning And HRM

Introduction In this competitive business environment where every business organization is trying to attract the customers of each other, it becomes essential for ...

Read More Tags: Australia Arlington Management Management University of New South Wales Management 
Next
Free plagiarismFree plagiarism check online on mobile
Have any Query?