Title: Effect of UV-Rays on human blood DNA
Natural impacts from UV radiation rely on upon wavelength, level of exposure, and span of exposure. Exposure of skin to germicidal wavelengths of UV light can deliver sunburn and skin cancer. Exposure of UV alone can make redness of skin, only just at high exposures. Exposure of the eyes to this UV radiation can create greatly excruciating irritation of the cornea and impermanent or changeless vision impedance, up to and including visual impairment in some cases. UV can harm the retina of the eye. Sensitiveness in the eyes and redness of facial skin typically happens inside 6?12 hours of introduction, with the indications and inconvenience enduring up to 48 hours. The non-melanoma skin growths basal and squamous cell carcinoma are not generally deadly but rather surgical treatment can be agonizing and result in scars. Non-melanoma skin diseases are most successive on those parts of the body that are normally presented to the sun, for example, ears, face, neck and lower arms. The finding that they are more continuous in open air than in indoor specialists recommends that the aggregated life-time introduction to UV radiation assumes a noteworthy part in the improvement of non-melanoma skin diseases. But this study is to find the effectiveness of ultraviolet radiation on the blood cells.
Approach for the study
For this study firstly we had to collect blood samples from a human being. Then the blood DNA is extracted by the Agarose Gel Electrophoresis and is purified by the electro elution method. Then DNA samples’ copies are made by the help of PCR technique. Next 10 samples of same blood DNA are prepared with different concentration of DNA were prepared. Those are then stored for the required time. In the meanwhile, laminar air flow is sterilized and is being prepared for the experiment to test the effect. The UV light of the laminar air flow is kept on and the glass lid is closed so that the ray does not affect someone or something else. Then after the required time the DNA samples are kept into thr3 laminar air for further observation.
Potential sources of data and information:
From Pub med among 1500; there are 4 relevant articles from where the information of “effect of UV rays on human blood” can be retrieved. In science hub there are 20 relevant articles about these topics.
- Comparative study of the amount of DNAsingle-strand breaks and alkali-labile sites in human peripheral blood lymphocytes exposed to 365 nm UV versus X-ray radiation
Author: Smetanina N.M. ; Pustovalova ?.V. ; Bushmanov A.Yu. ; Osipov A.N.
Published in: Saratov Journal of Medical Scientific Research, 01 December 2013, Vol.9(4), pp.791-795
- The formation of DNA single-strand breaks and alkali-labile sites in human blood lymphocytes exposed to 365-nm UVA radiation
Author: Osipov, Andreyan N. ; Smetanina, Nadezhda M. ; Pustovalova, Margarita V. ; Arkhangelskaya, Ekaterina ; Klokov, Dmitry
Published in: Free Radical Biology and Medicine, August 2014, Vol.73, pp.34-40
- The effect of donor age on the processing of UV-damaged DNA by cultured human cells: reduced DNA repair capacity and increased DNA mutability
Author: Moriwaki, S ; Ray, S ; Tarone, R E ; Kraemer, K H ; Grossman, L
Published in: Mutation research, 18 October 1996, Vol.364(2), pp.117-23
Czapla-Masztafiak, J., Szlachetko, J., Milne, C. J., Lipiec, E., Sá, J., Penfold, T. J., ... & Kwiatek, W. M. (2016). Investigating DNA radiation damage using X-ray absorption spectroscopy. Biophysical journal, 110(6), 1304-1311.
Rich, E. A., Elmets, C. A., Fujiwara, H., Wallis, R. S., & Ellner, J. J. (1987). Deleterious effect of ultraviolet-B radiation on accessory function of human blood adherent mononuclear cells. Clinical and experimental immunology, 70(1), 116.
Breuer, J., Schwab, N., Schneider?Hohendorf, T., Marziniak, M., Mohan, H., Bhatia, U., ... & Meuth, S. G. (2014). Ultraviolet B light attenuates the systemic immune response in central nervous system autoimmunity. Annals of neurology, 75(5), 739-758.
Lee, C. H., Wu, S. B., Hong, C. H., Yu, H. S., & Wei, Y. H. (2013). Molecular mechanisms of UV-induced apoptosis and its effects on skin residential cells: the implication in UV-based phototherapy. International journal of molecular sciences, 14(3), 6414-6435.