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PC1002 Water Diuresis And Urinalysis

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Question

Pre-Lab Flow Chart: Signed on the day by academic staff, a basic description of how the experiment will be conducted. Can be a ½-1 page before the remaining items below. 

Part 1.

4 graphs or 2 graphs (if you can show a water line and saline line on one graph). 
  • water/urine flow rate vs. time
  • saline/urine flow rate vs. time
  • water/urine osmolality vs. time
  • saline/urine osmolality vs. time
On each osmolality graph show the morning sample (it will not have a corresponding “time” value, so plot against time = 0 minutes). Provide a brief written description of each graph.

Part II.

You need to put in the tables with appropriate descriptions in the Results.

Discussion

Part I. Water diuresis

Make sure you have answered the following questions in the discussion (in question and answer format e.g.
 
Water Treatment
  1.  The consumption of water causes
The questions does not have to appear in the report, as long as it is clear that you are answering each question (use headings, #s or paragraph breaks).

Water treatment

1. How does the consumption of water affect the ECF volume and ECF osmolarity?
2. How did the resulting change in ECF osmolarity affect ADH release? (include the receptors involved in detecting the alteration and where the hormone is released from, and whether there was an increase or decrease in hormone level)
3. What are the physiological actions of ADH?
4. How did treatment with water affect urine flow rate and urine osmolarity?
5. The change in ECF osmolarity is also detected by macula densa cells. What effect does this change have on the amount of aldosterone that is released?
6. What is the function of aldosterone on the distal convoluted tubules and collecting duct?

Saline treatment

1. How did the treatment with saline affect the ECF volume and ECF osmolarity?
2. How did the resulting change in ECF volume affect the amount of ANP released?
3. How did treatment with saline affect urine flow rate and urine osmolarity?
4. What are the physiological actions of ANP?

Morning Sample

1. How did the osmolarity of the morning sample differ from the other samples collected from subject 1 (after drinking water)?
2. What causes the morning urine sample to be different from other samples collected during the day? i.e. think about how the levels of ADH and Aldosterone would change during the night.

Part II. Urinalysis

Compare the Unknowns with the controls. What is wrong with the people with the Unknown samples – Why?
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