Role of citrate in blood coagulation
The use of tri-sodium citrate in case of anticoagulation of blood is a very old method. The property registered by the ability to combine with calcium ions in the blood. So they become unavailable for participation in various important points of the pathway of coagulation (Apan.com.au, 2015). Citrate is to be added, in the form of sodium citrate or acid-citrate-dextrose (Cohen, 2015).
Mann KG, Whelihan MF, Butenas S, Orfeo T. (2007) Citrate anticoagulation and the dynamics of thrombin generation. J. Thromb Haemost; 5: 2055–61.
This article was chosen because it provided an in depth knowledge of the influence of citrate anticoagulant on blood and plasma assays. In three ways blood was anticoagulated. One was into citrate, one was into CTI and the other into citrate with CTI. Plasma was prepared with each regime. Functional analysis was done. In all cases reaction dynamics was altered.
Whole blood anticoagulation:
Stief, Thomas (2013). Reactive oxygen species generation in diluted whole blood anticoagulated by citrate, EDTA, or heparin Hemostasis Laboratory6.2/3 : 155-173.
This article tells us about Reactive Oxygen Species (ROS). 100 microlitre of blood was incubated with citrate. ROS generation was measured in laminator. Citrate blood had roughly 1420 RLU per sample, in time to 50 % of maximum 35 minutes. In 42 minutes it was 560 RLU per sample. Thus we can say that blood should be freshly analysed.
Anticoagulation for renal replacement therapy:
Straaten, H. M. O., Kellum, J. A. and Bellomo (2011) Clinical review: Anticoagulation for continuous renal replacement therapy-heparin or citrate? Crit Care, 15(1), p.202
This article identifies the capability of citrate anticoagulation to avoid patient’s bleeding risk. It highlights the benefits of citrate regarding better kidney survival of patient. Going by the view of Tolwani and Wille (2012), it emphasizes on controlling the risks of citrates to achieve its full advantages.
Apan.com.au, (2015). The Use of Citrate as an Additive in Blood Collection Tubes. [online] Available at: https://www.apan.com.au/the-use-of-citrate-as-an-additive-in-blood-collection-tubes [Accessed 19 Jun. 2015].
Cohen, B. (2015). How Does the Anticoagulant Citrate Work? | eHow. [online] eHow. Available at: https://www.ehow.com/how-does_5234167_anticoagulant-citrate-work_.html [Accessed 19 Jun. 2015]
Tolwani, A. and Wille, K. (2012) Regional Citrate Anticoagulation for Continuous Renal Replacement Therapy: The Better Alternative? American Journal of Kidney Diseases,59(6), pp.745-747.