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Chemistry
Answer: Robinsonia genus plants are terrestrial and indigenous to the Juan Fernández Islands, comprising of 8 species. Phylogenetic studies have suggested them to be nested within the Senecio genus, though these two genus are morphologically distinct (Pelser et al. 2010). The current study attempts to understand the phylogenetic relations between Robinsonia genus and Senecio genus. Two plant species from th...
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Answer: Introduction Obesity is a killer epidemic, and its rates are alarming in the American state. Besides, obesity causes health issues associated with health problems that may be fatal such as cardiovascular disease, type 2 diabetes, some cancers, and elevated cholesterol. Hence the necessity to analyze behavioral changes, resources available, prevention, and intervention strategies to improve obesity health issues. There are behavior ch...
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Question: Discuss about the Magnetic Particles For The Separation Purification. Answer: Introduction The given study titled, “Human Breast Cancer: Correlation of Relapse and Survival with Amplification of the HER-2lneu Oncogene”, aimed to identify the role of genes in the biological behaviour or pathogenesis of human breast cancer. During the period in which the given study was undertaken the evidences relating the proto...
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Question: Discuss about the Photorhabdus luminescens Bacteria. Answer: Introduction Photorhabdus luminescens bacteria, belongs to the family Enterobactriaceae, and acts as a lethal pathogen for the insects. The bacteria reside in the gut of the nematode of family Heterorhabditidae, in a symbiotic association (Murfin et al, 2012). The life cycle of these bacteria shows a strange switch between virulent and the avirulent form,...
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Question: Discuss about the Review of Ebora Viral Disease. Answer: Ebola Viral Disease EBOV is the agent responsible for the transmission of Ebola disease. This disease has spawned several epidemics the past four decades. For instance, in 2014, Ebola became an epidemic after spreading from Africa to other continents. The fact that the there was no effective treatment confused the whole world. This virus has a relatively uniq...
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Question: Discuss about the Structure and Assembly of Secreted Mucins. Answer: Introduction: This coral reef cnidarian is radially symmetrical around the axis that is formed around the mouth, or the oral axis and the opposite end which is the aboral axis or the base. It has an opportunistic feeding habit and its radial symmetry allows it to detect prey and predators from any direction. It has a polyp form that has a cylindri...
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Question: Discuss about the Application of Genetically Modified Organisms. Answer: Genetically modified organisms are the result of laboratory process in which D.N.A from one species is extracted and incorporated into another desired organisms. The foreign genes are to be taken from the bacteria, viruses mainly. The organisms that are produced as a result of genetic modifications are referred to as the transgenic organisms. ...
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Question: Discuss About The Polymerase Chain Reaction Animal Science? Answer: Introduction The polymerase chain reaction (PCR) is a popular method used in molecular biology under the branch of sub atomic science. It duplicates the DNA double helix at an exponential rate. Here the DNA polymerase replicates the DNA double helix in a chain wise manner from the 5’ to 3’ direction and hence known as PCR. The concept of PC...
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Question: Describe about the Molecular Biology for Surrogate Marker for Endoscopic. Answer: 1. The conditional and inducible gene knockout is a type of technique which is used for the elimination of a particular gene from a certain tissue like the kidney and liver. The use of this technique is in the study and determination of the function of specific individual genes of the living organisms. This process is different from the traditio...
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Question: Discuss about the Molecular Biology for Neurodegenerative Disease. Answer: Introduction In this research topic the role of autophagy in the neurodegenerative disease is investigated taking the mouse model C57BL/6j which must contain the GFP-LC3 transgene and also expresses GFP—LC3. In this study an outline of the rationale for using mouse model C57BL/6j is performed. The light chain 3 (LC3) is a microtubul...
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Question: Discuss about the A-amylase Gene using Polymerase Chain Reaction. Answer: Introduction Bacillus subtilis is a Gram-positive, rod-shaped and flagellated bacterium that are natural inhabitants of soil and vegetation as well as our gastrointestinal tract. It has a circular chromosome like most of the prokaryotes with approximately 4214.8 Kb (Kunst et al., 1997) coding for more than 4000 proteins (Kobayashi et al., 2003)...
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Question: Discuss about the Introduction to Molecular Vaccinology. Answer: The vaccine developed against smallpox is vaccinia virus. Experiments were conducted with this “replication competent virus” and the results showed effectiveness. Therefore, with further development of Recombinant DNA Technology, recombinant vaccinia vaccines were generated that express the “desired foreign genes” (Monath, 2005). This v...
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Question: RNAi is a fairly new tool that molecular biologist use to inhibit gene expression. RNAi can inhibit gene expression in two ways. Please describe the principle of RNAi and the two ways in which RNAi can inhibit gene expression. Answer: Principle of RNAi RNAi refers to RNA interference. It is a process of gene silencing, which is sequence-specific and occurs at the post-transcriptional level (Allison, 2007). It involve...
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Question: Discuss the purpose or aim of the experiment. Answer: To carry out blue white screening for confirmation of ligation of PCR products into desired cloning vector Material: Ecoli strains (DH5α), PCR product, T4 DNA ligase enzyme, Luria Bertanni agar, Petri plates, ice, double distilled water, X-Gal, pipettes, PCR master mix, desired restriction endonucleases (Nybo, 2010). Procedure: Gene of interested is amplified...
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Question: Analyze the effect of various transcriptional regulatory proteins. Answer: An operon is a particular portion of genetic material that acts as a functioning unit and is composed of a cluster of genes that are controlled under a single promoter. In the process of transcription, there are two types of operons and those are, lac-operons or inducible operons and repressible operons (Dillon & Dorman, 2010). A lac-operon is a...
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