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Chemistry
Question: Describe about the Chromatographic Purification? Answer: Purification of Soluble His-Tagged Proteins The ClpA is the protein of our interest is an ATP dependend chaperon and regulatory component of ClpAP protein basically expressed in E.coli cells. This expression of recombinant protein could be analysed by presence of small size of amino acids i.e. 6 histidine tag which will be conjugated to its C or N terminus of...
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Question: Describe about the Affective Commitment to Change and Innovation Implementation Behavior? Answer: Introduction There was a time when Information Technology was considered as a secondary function in the organization. With spread of technology, Information System has emerged as important business function. There was a time when Information Systems were used primarily by large organizations only. With time, the cost o...
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Question: Discuss the role of DNA supercoiling in genome structure and regulation? Answer: DNA (Deoxyribonucleic acid) is the molecule carrying the genetic instructions that are needed for the functioning, development and reproduction of al living organisms and many viruses. DNA supercoiling is the under- or over-winding of the DNA strand and is the expression of the strain on the strand (Corless, 2014). The present paper discusses t...
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Questions: 1. Can you clarify and describe the connection between evolution and the inheritance of genetic material.2. You book speaks of an experiment by Frederick Griffith. How did Griffith’s experiment change the direction of molecular biology? Be specific and fully explain your answer. 3. Now let's move forward and think about the Medel experiments. Discuss the connection between Mendel's results and the process of meiosis. An...
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Question: Discuss the role of restriction enzymes as a defense mechanism by bacteria. Answer: Restriction modification system can be considered as the primitive immune system of bacteria and due to the diversity and prevalence across the prokaryotic system helps in increasing the effectiveness of defense mechanism against the other genomes. There are mainly two type of restriction enzymes are involved in this particular case of modif...
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Question: Discuss at least two different kinds of probes and how they can be used to screen libraries. Answer: The probes that are used to screen a DNA library are RNA or DNA fragments that detect the nucleotide sequences in a sample. One type of probe is Molecular bacons which are composed of 25 nucleotides. The five nucleotides at each end are complementary to each other and 15 nucleotides of the middle portion are complementary to t...
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Question: Determine how a promoter or other regulator region of a gene functions. Answer: The researchers attach a gene to the regulatory sequence of another gene to analyze the expression of that gene is known as the reporter gene. A reporter gene cat is derived from the E. coli Tn9 species that give the product Chloramphenicol acetyltransferase (CAT), which is a very effective reporter to study in vitro assays. With the aid of thin...
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Question: Analyze the effect of various transcriptional regulatory proteins. Answer: An operon is a particular portion of genetic material that acts as a functioning unit and is composed of a cluster of genes that are controlled under a single promoter. In the process of transcription, there are two types of operons and those are, lac-operons or inducible operons and repressible operons (Dillon & Dorman, 2010). A lac-operon is a...
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Question: Discuss the purpose or aim of the experiment. Answer: To carry out blue white screening for confirmation of ligation of PCR products into desired cloning vector Material: Ecoli strains (DH5α), PCR product, T4 DNA ligase enzyme, Luria Bertanni agar, Petri plates, ice, double distilled water, X-Gal, pipettes, PCR master mix, desired restriction endonucleases (Nybo, 2010). Procedure: Gene of interested is amplified...
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Question: RNAi is a fairly new tool that molecular biologist use to inhibit gene expression. RNAi can inhibit gene expression in two ways. Please describe the principle of RNAi and the two ways in which RNAi can inhibit gene expression. Answer: Principle of RNAi RNAi refers to RNA interference. It is a process of gene silencing, which is sequence-specific and occurs at the post-transcriptional level (Allison, 2007). It involve...
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