The mitosis which is generally the division of the cells is divided into five different phase which is the prophase, prometaphase, metaphase, anaphase and the last phase is known as the telophase. Initially in the metaphase it is observed that all the chromosomes are present in a single line of the cell. This arrangement of the chromosomes makes it easy to pull apart the two identical daughter cells in the next phase which is the anaphase. Metaphase is also observed in the process of where meiosis. In the process of meiosis, it is observed that there is a different type of nuclear division that help in the production of egg cells and the haploid sperms.  In this phase the chromosomes which have been already replicated from the parents are joined at a central point which is termed as centromere or the sister chromatid. There is a checkpoint which is also known as the metaphase checkpoint and the plane where the chromosomes are aligning is known as the equatorial plane. The line upon which the chromosomes are situated is an imaginary line.

The metaphase must be taken care as in this stage the separation of the two chromatids occurs. The two sister chromatids which are joint at a point are separated from each other. The cells wait for this metaphase as all the chromatids are attached with the mitotic spindle till this phase. It is one of the important stage where the separation need to occur carefully so that no sister chromatids remains attached and are separated properly.