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BHS025-6 Molecular And Diagnostic Microbiology

Question

Answered

Question:

Instructions To Candidates

  • You must answer TWO questions in total. You must choose one question from Section A and you must answer the compulsory question in Section B.
  • This is a closed book examination. No calculators and no additional materials (including dictionaries) are allowed in the examination room.
  • Write your answers in the answer books provided. Please use one answer book for each of your answers.
  • Documents provided with the exam paper – NONE

Section A (Answer one question, 50% of total mark)

  1. Discuss the impact of chromogenic agars in relation to Diagnostic Microbiology and public health. Discuss your thoughts on the sensitivity of chromogenic agars compared to conventional media (100%).
  2. Discuss ‘is automation good or bad’ and whether you will support total laboratory automation (TLA). In your answer, comment on the impact of the development of various automation including molecular analysis in clinical Microbiology since 1980 (100%).

A -21-year old heterosexual man came to see his general practitioner with a 24 hrs of painful dysuria and a thin yellowish penial discharge. A direct

Staining smear of a urethral swab shows excessive pus cells and scattered intra-and extra-cellular organisms. Discuss in detail how would you diagnose this case (100%).

Section B

Compulsory question (50% of total mark)

Explain the principle and practice of MALDI-TOF mass spectrometry (50%).

Discuss how it contributes to reducing turnaround time in a clinical laboratory (50%).

BHS025-6 Molecular And Diagnostic Microbiology

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Tellus molestie nunc non blandit massa enim nec dui. Tellus molestie nunc non blandit massa enim nec dui. Ac tortor vitae purus faucibus ornare suspendisse sed nisi. Pharetra et ultrices neque ornare aenean euismod. Pretium viverra suspendisse potenti nullam ac tortor vitae. Morbi quis commodo odio aenean sed. At consectetur lorem donec massa sapien faucibus et. Nisi quis eleifend quam adipiscing vitae proin sagittis nisl rhoncus. Duis at tellus at urna condimentum mattis pellentesque. Vivamus at augue eget arcu dictum varius duis at. Justo donec enim diam vulputate ut. Blandit libero volutpat sed cras ornare arcu. Ac felis donec et odio pellentesque diam volutpat commodo. Convallis a cras semper auctor neque. Tempus iaculis urna id volutpat lacus. Tortor consequat id porta nibh.

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BIOL41-Microbiology

Questions: 1.  What would the consequence be of raising the starting pH of EMB agar to 7.8 (normal pH = 7ish)?  Would it alter the selective nature of the media, the differential nature of the media or both?  Would it be more or less sensitive (more = detects coliforms more easily, l ...

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