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CRN15456 :clinical and applied sciences

D

Effects of Hydrogen Peroxide ...

Dr Andrew Paterson Experiment 1 : Effects of H 2 O 2 on viability of SHSY 5 Y neuronal cells determined by Trypan Blue dye exclusion . (a) 24 - well plates were ...

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D

Principles of Human Physiology ...

HRP treated Cells 1 1.987 1.528 1.353 0.958 0.812 0.714 0.625 HRP treated Cells 2 2.011 1.601 1.42 1.058 0.755 0.74 0.572 HRP treated Cells 3 1.923 1.791 1.27 1.053 0.799 0.683 0.655 mean 1.974 1.640 ...

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D

Curve and Cell Samples ...

Experiment 2 Data for HRP Standard Curve and Cell Samples Plate 1: Standards HRP (µg/ml) Absorbance at 45 0 nm 0 0.127 0.131 0.126 0.124 0.110 0.12 ...

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D

Range of Key Molecular ...

02/11/2020 1 Dr Andrew Paterson How is the module assessed? The assessment for this module is an integrated write up of data from a series of experiments (comprises 100% of module assessment), to be ...

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More CRN15456 clinical and applied sciences: Questions & Answers

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Effects of Hydrogen Peroxide

Dr Andrew Paterson Experiment 1 : Effects of H 2 O 2 on viability of SHSY 5 Y neuronal cells determined by Trypan Blue dye exclusion . (a) 24 - well plates were seeded with 5 x 10 5 cells/well and incubated overnight at 37 ºC . (b) A fresh stock of H 2 O 2 was prepared in serum free media . (c) D ilutions o ...

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Principles of Human Physiology

HRP treated Cells 1 1.987 1.528 1.353 0.958 0.812 0.714 0.625 HRP treated Cells 2 2.011 1.601 1.42 1.058 0.755 0.74 0.572 HRP treated Cells 3 1.923 1.791 1.27 1.053 0.799 0.683 0.655 mean 1.974 1.640 1.348 1.023 0.789 0.712 0.617 Control Cells 1 2.705 1.404 1.13 0.877 0.728 0.463 0.372 Control Cells 2 2.956 1.427 1.236 0.905 0.713 0.527 0.38 Control Cells 3 2.628 1.236 1.18 0.941 0.662 0.561 0.34 ...

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Curve and Cell Samples

Experiment 2 Data for HRP Standard Curve and Cell Samples Plate 1: Standards HRP (µg/ml) Absorbance at 45 0 nm 0 0.127 0.131 0.126 0.124 0.110 0.127 0.124 0.132 0.123 0.127 0.156 0.184 0.157 0.166 0.164 0.165 0.162 0.220 0.150 0.196 0.169 0.313 0.193 0.191 0.158 0.170 0.207 0.241 0.186 0.143 0.19 ...

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Range of Key Molecular

02/11/2020 1 Dr Andrew Paterson How is the module assessed? The assessment for this module is an integrated write up of data from a series of experiments (comprises 100% of module assessment), to be written in the style of a journal article. 1 2 02/11/2020 2 Aim To determine the effects of hydrogen peroxide (H 2 O 2 ) on cell viability, to determine peroxidase (HRP) uptake into cells and to dete ...

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