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###### BIOL3100 Microbiology

1 Context you will play the role of a microbiologist researching a topic or a problem of significant interest and to propose potential solutions in a written report/scientific review article to be ...

Course

BIOL3100

Type

Assignment

Subject

Biology

University

The University of Newcastle

Season

Fall

## Questions:

1. Most laboratory experiments require the preparation of Buffer solutions. Therefore, stock solutions of the different buffer components are prepared. The following list shows the substances, the respective molecular weights, and the stock solution you should prepare.

Calculate the amount of the substance you have to weigh in

2. Using the Stock solutions calculated above, you have to prepare the following Buffer for your experiment.

Calculate the respective volumes for a 150m1 solution.

3. As a next step you plan to prepare a liposome (lipid vesicle) solution in the above prepared Buffer using Polar Lipid Extract (PIE) from Escherichio Coll. How much E. coil PLE do you have to weigh in to prepare 125011 of a 20mg/m1 solution?

Considering the phospholipid profile of E. coil PLE, what are the final molarities of the respective lipid species in the solution?

### BIOL10212 Biochemistry

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Tellus molestie nunc non blandit massa enim nec dui. Tellus molestie nunc non blandit massa enim nec dui. Ac tortor vitae purus faucibus ornare suspendisse sed nisi. Pharetra et ultrices neque ornare aenean euismod. Pretium viverra suspendisse potenti nullam ac tortor vitae. Morbi quis commodo odio aenean sed. At consectetur lorem donec massa sapien faucibus et. Nisi quis eleifend quam adipiscing vitae proin sagittis nisl rhoncus. Duis at tellus at urna condimentum mattis pellentesque. Vivamus at augue eget arcu dictum varius duis at. Justo donec enim diam vulputate ut. Blandit libero volutpat sed cras ornare arcu. Ac felis donec et odio pellentesque diam volutpat commodo. Convallis a cras semper auctor neque. Tempus iaculis urna id volutpat lacus. Tortor consequat id porta nibh.

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### BIOL3100 Microbiology

1 Context you will play the role of a microbiologist researching a topic or a problem of significant interest and to propose potential solutions in a written report/scientific review article to be presented to the scientific community. 2 Task Your report/scientific review must be developed ...

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