Chemical Engineering Assignment Help
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Sample Question & Answer Of Chemical Engineering Assignment Help
CHEN20051 Chemical Engineering Optimisation
Question:
You are a process engineer working in a major multinational company. There is within the company a desire to make petrol as a product in the most environmentally-friendly way possible, using a biological process. In addition, part of your company generates lignocellulosic hydrolysate in large quantities as a by-product of a process. You need to use this as a feedstock for your process.
Your company has a background in biological processes using the bacterium Escherichia coli. Your process must use this. In addition, the organism that you engineer must be used in an immobilized form to retain biomass in the bioreactor.
Your company has a large bioprocess/fermentation development division, so you do not need to discuss this. You need to focus on how you will engineer the organism (E. coli) to make your product from the stated feedstock.
Answer:
Introduction
From a biotechnology viewpoint, fatty acids are energy sufficient and are thus integrated into intracellular lipid. Lately, fatty acid metabolism has got a considerable focus as a pathway for generating high-density transference gasses. Lignocellulosic constituents comprise mostly of three polymers: hemicellulose, cellulose, and lignin (Kim, Ximenes, Mosier and Ladisch 2011). The Lignocellulosic feedstock requires forceful pretreatment to produce a substrate simply hydrolysable by viable cellulolytic enzymes or by enzyme generating microbes, to release sugar required for fermentation (Rumbold et al. 2010). Cellulose is a key constituent of the biomass which is a polymer of β-D-glucopyranose bits connected through β-(1, 4) glycosidic bonds with known polymorphs. Read More ...
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