BIOL2071 Biology

You are newly hired as a staff scientist at a start-up pharmaceutical company, Vertex Pharmaceuticals. The project you are assigned to concerns the life cycle and biology of Kaposi's sarcoma-associated herpesvirus (KSHV), a double-stranded DNA virus that infects humans. It is one of seven known cancer-causing viruses in humans, and there is no known cure as of this date. You are asked to use your expertise in molecular biology to study the K1 gene from this virus and ultimately devise a strategy to determine if several other viral proteins can bind to the K1 protein. The hope is that by understanding what the K1 viral protein binds to, we can start to get an idea of what this viral gene is responsible for, and thus better understand the biology of the virus.The virus codes for almost 100 genes, but your research team is interested in determining whether the K1 protein can bind to the viral ORF23, ORF34, and ORF42 proteins specifically. You are given a list, along with sequence information for these genes below.Viral Gene NameCDSK) Identify the minimum number of constructs that need to be made for you to answer this research question using the general class of assays we focused on in 2071. Assume you have no finished constructs to begin with, and antibodies specific to these viral proteins are unavailable. Please use the table below and indicate what would need to be engineered into the MCS (description of construct), and why you are generating the construct (rationale for use). Three empty rows are provided, but you can generate less or more. Description of ConstructRationalefor Use2.(2 points) You wish to clone the full-length K1 protein coding sequence into a vector using XbaI at the 3’ end and EcoRI at the 5’ end of the gene. Devise a set of 25 nucleotide long primers to amplify the K1 gene to accomplish this task.