NEUR302 Applied Neuroscience
Task
The Mind Project’s Virtual Cocaine Lab
Lab Book
Instructions: This is your lab book. The lab itself is open in another window. As you go about the lab completing tasks, you will need to answer specific questions and to record your observations. Use this lab book as a reference when the time comes to publish the results of this experiment.
Desk area
1. What are the two phases of motivated behavior?
a)
b)
2. What is known about the effects of cocaine on dopamine levels in the brain?
3. What is the hypothesis being tested in this experiment?
4. If the hypothesis is true, then what should you observe?
5. What is the name of the method used to measure dopamine levels?
6. What is the formula for computing how much anesthesia to administer?
7. Where will you inject the rat with the anesthesia?
Prep area
8. Based on the weight of the rat you selected, how much anesthesia should you extract?
Surgery area
9. What is the function of the canula?
10. What is the name of the device that allows us to implant electrodes in precise locations?
11. Which electrode measures dopamine levels?
Experiment area
12. What is the function of the telemetry pack?
13. What behavior are you looking for when the rat is seeking cocaine?
14. What behavior(s) do you observe when the rat is not seeking cocaine?
15. Do you observe any change in dopamine levels that is consistent with what is known about cocaine being consumed? If so, describe those changes.
16. Do you observe any changes in dopamine levels while the rat is engaged in cocaine seeking behavior? If so, describe those changes.
17. Do the results of this experiment support the hypothesis? Explain why or why not.
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