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Differentiation Of Bacterial Strains Through Selective Media And Microscopy

BSC 2891 Microbiology and Cell Culture

6 Pages / 1,371 Words Published On: 17-08-2020

Part 1: Outline how you would differentiate the bacterial strains listed below using selective media and microscopy.

Details of the following selective media and stains: check the protocol log book as this contains info that will help you.
Bacterial strains
Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, Proteus mirabilis, Enterobacter cloacae, Eschericha coli
Growth on TSA plates, stabs, broths and slope cultures, SIM stab culture including use of Kovak’s reagent, EMB plates, PEA plates, and fermentation assays testing for acid and gas production when using glucose, lactose and sucrose as carbon sources. Gram staining, endospore staining and testing for catalase activity.
You have an unknown bacteria with the following characteristics: Gram negative short rod, H2S producer and ferments sucrose to give acid and gas.
Which bacteria do you conclude this unknown to be? Outline your reasons and explain what experiments you would do to confirm your identification giving details of results you would expect.

Part 2: You have a pear showing signs of brown rot caused by a bacterial pathogen. Outline the stages and techniques used to carry out a Koch’s postulate to identify the causal agent of the bacterial rot on the pear. Which bacterial pathogen is causes this disease?

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